Recombinant L1CAM anticorps

N° du produit ABIN7072598

L’anticorps Souris Monoclonal anti-L1CAM a été validé pour WB, IHC, FACS, IF et IP. Il convient pour détecter L1CAM dans des échantillons de Humain.

Aperçu rapide pour Recombinant L1CAM anticorps (ABIN7072598)

Antigène: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp L1CAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: L1-14-10

Détail du produit anti-L1CAM anticorps

Fonction: Anti-CD171 [L1-14.10], Mouse IgG1, kappa

Specificité: This antibody is specific for the ectodomain (amino acids M1 to E1123) of human L1, and recognises an epitope in the third Ig domain. L1 is over-expressed in a range of tumours, including neuroblastomas, renal carcinomas, ovarian and endometrial carcinomas, and melanomas. This antibody does not cross-react with human CHL1.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The specificity of this antibody has been confirmed in flow cytometric (Schafer et al, 2012, Huszar et al, 2006) and Western blot analysis (Huszar et al, 2006, Wolterink et al, 2010). This antibody has been shown, when incubated with SKOV3ip human ovarian carcinoma cells, to efficiently and sustainedly block extracellular signal-regulated kinase (Erk) phosphorylation (Gast et al, 2008). This antibody has been used in immunohistochemical staining for L1 expression, using paraffin-embedded human ovarian carcinoma tissue sections (Wolterink et al, 2010, Knogler et al, 2007) and a panel of human tumour tissue sections, including benign and malignant tumours of the female genital tract (Huszar et al, 2006), and renal cell carcinoma, schwannoma, gastric conventional GIST, pancreatic neuroendocrine tumor and paraganglioma (Inaguma et al, 2016). This antibody, when administered to SCID mice in combination with cytostatic drugs, efficiently reduces the growth of subcutaneously grown Colo357 or SKOV3ip tumors (Schafer et al, 2012).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur L1CAM

Antigène: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Autre désignation: CD171

Sujet: L1CAM, 14.10, L1, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

UniProt: P32004

Pathways: Synaptic Membrane