BUB1 anticorps (AA 951-1085)

N° du produit ABIN706541

Détail du produit anti-BUB1 anticorps

Antigène: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Épitope: AA 951-1085

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BUB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain, Souris

Homologie: Rat,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Bub1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur BUB1

Antigène: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Autre désignation: Bub1 (BUB1 Produits)

Synonymes: anticorps bub1a, anticorps bub1l, anticorps hbub1, anticorps xbub1, anticorps BUB1A, anticorps BUB1L, anticorps hBUB1, anticorps AL022991, anticorps Bub1a, anticorps C80208, anticorps D2Xrf87, anticorps BUB1 mitotic checkpoint serine/threonine kinase, anticorps BUB1, mitotic checkpoint serine/threonine kinase, anticorps BUB1, anticorps bub1, anticorps Bub1

Sujet:

Synonyms: BUB1A, BUB1L, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1

Background: Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

ID gène: 699

UniProt: O43683