RAD1 anticorps

N° du produit ABIN7112556

Cet anticorps anti-RAD1 est un anticorps Lapin Polyclonal détectant RAD1 dans WB, ELISA, IHC et IP. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour RAD1 anticorps (ABIN7112556)

Antigène: RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit anti-RAD1 anticorps

Fonction: RAD1 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: RAD1 homolog(S. pombe)

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur RAD1

Antigène: RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))

Autre désignation: RAD1

Sujet:

Synonyms: Cell cycle checkpoint protein RAD1 (hRAD1)|Rad1-like DNA damage checkpoint protein|RAD1|REC1

Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER). The 9-1-1 complex stimulates DNA polymerase beta(POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I(LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks(DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

Poids moléculaire: 32 kDa

ID gène: 5810

UniProt: O60671

Pathways: M Phase