CARM1 anticorps (AA 443-608)

N° du produit ABIN7112899

Cet anticorps Lapin Polyclonal détecte spécifiquement CARM1 dans WB, ELISA, IHC et IF. Il présente une réactivité envers Humain, Souris, Rat et Singe.

Aperçu rapide pour CARM1 anticorps (AA 443-608) (ABIN7112899)

Antigène: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CARM1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-CARM1 anticorps

Épitope: AA 443-608

Fonction: CARM1 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: 443-608aa

Immunogen: coactivator-associated arginine methyltransferase 1

Isotype: IgG

Information d'application

Indications d'application: WB: 1:1000-1:5000, IHC: 1:50-1:500, IF: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur CARM1

Antigène: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Autre désignation: CARM1

Sujet:

Synonyms: Histone-arginine methyltransferase CARM1|Coactivator-associated arginine methyltransferase 1|Protein arginine N-methyltransferase 4|CARM1|PRMT4

Background: CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTMsites in cells themselves.

Poids moléculaire: 63-66 kDa, 55 kDa

ID gène: 10498

UniProt: Q86X55

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation