Cullin 4A anticorps (AA 460-759)

N° du produit ABIN7113117

Cet anticorps anti-Cullin 4A est un anticorps Souris Monoclonal détectant Cullin 4A dans WB, ELISA, IHC, IF et IP. Adapté pour Humain, Souris, Rat et Singe.

Aperçu rapide pour Cullin 4A anticorps (AA 460-759) (ABIN7113117)

Antigène: Cullin 4A (CUL4A)

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cullin 4A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 5A7

Détail du produit anti-Cullin 4A anticorps

Épitope: AA 460-759

Fonction: CUL4A antibody

Purification: Protein A+G purification

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: 460-759aa

Immunogen: cullin 4A

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur Cullin 4A

Antigène: Cullin 4A (CUL4A)

Autre désignation: CUL4A

Sujet:

Synonyms: Cullin-4A (CUL-4A)|CUL4A

Background: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled(H3.1) and replication-independent(H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.

Poids moléculaire: 88 kDa, 77 kDa

ID gène: 8451

UniProt: Q13619