DGCR8 anticorps (AA 430-773)

N° du produit ABIN7113479

L’anticorps Souris Monoclonal anti-DGCR8 a été validé pour WB, ELISA et IP. Il convient pour détecter DGCR8 dans des échantillons de Humain et Souris.

Aperçu rapide pour DGCR8 anticorps (AA 430-773) (ABIN7113479)

Antigène: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DGCR8 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 2G8

Détail du produit anti-DGCR8 anticorps

Épitope: AA 430-773

Fonction: DGCR8 antibody

Purification: Protein A+G purification

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: 430-773aa

Immunogen: DiGeorge syndrome critical region gene 8

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:2000, IP: 1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur DGCR8

Antigène: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Autre désignation: DGCR8

Sujet:

Synonyms: Microprocessor complex subunit DGCR8|DiGeorge syndrome critical region 8|DGCR8|C22orf12|DGCRK6

Background: Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA(miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts(pri-miRNAs) to release precursor miRNA(pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs(PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer(of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding(PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine(m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing(PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal(By similarity).

Poids moléculaire: 120 kDa

ID gène: 54487

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways