EIF4A3 anticorps

N° du produit ABIN7113965

L’anticorps Lapin Polyclonal anti-EIF4A3 a été validé pour WB, ELISA et IP. Il convient pour détecter EIF4A3 dans des échantillons de Humain et Souris.

Aperçu rapide pour EIF4A3 anticorps (ABIN7113965)

Antigène: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4A3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-EIF4A3 anticorps

Fonction: EIF4A3 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: eukaryotic translation initiation factor 4A, isoform 3

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur EIF4A3

Antigène: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Autre désignation: EIF4A3

Sujet:

Synonyms: Eukaryotic initiation factor 4A-III (eIF-4A-III, eIF4A-III)|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD box protein 48|Eukaryotic initiation factor 4A-like NUK-34|Eukaryotic translation initiation factor 4A isoform 3|Nuclear matrix protein 265 (NMP 265, hNMP 265)|Eukaryotic initiation factor 4A-III, N-terminally processed|EIF4A3|DDX48|KIAA0111

Background: ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X(and probably other apoptotic genes), specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S), the function is different from the established EJC assembly. Involved in craniofacial development.

Poids moléculaire: 47 kDa

ID gène: 9775

UniProt: P38919