NEK2 anticorps

N° du produit ABIN7116883

Cet anticorps Lapin Polyclonal détecte spécifiquement NEK2 dans WB, ELISA et IHC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour NEK2 anticorps (ABIN7116883)

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-NEK2 anticorps

Fonction: NEK2 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: NIMA(never in mitosis gene a)-related kinase 2

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur NEK2

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Autre désignation: NEK2

Sujet:

Synonyms: Serine/threonine-protein kinase Nek2|HSPK 21|Never in mitosis A-related kinase 2 (NimA-related protein kinase 2)|NimA-like protein kinase 1|NEK2|NEK2A|NLK1

Background: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation(essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGO1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly(PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis(PubMed:25704143). Involved in the regulation of centrosome disjunction(PubMed:26220856). Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.

Poids moléculaire: 48 kDa

UniProt: P51955

Pathways: M Phase