POLR3A anticorps (AA 1-200)

N° du produit ABIN7117679

L’anticorps Lapin Polyclonal anti-POLR3A a été validé pour ELISA et WB. Il convient pour détecter POLR3A dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour POLR3A anticorps (AA 1-200) (ABIN7117679)

Antigène: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR3A est non-conjugé

Application: ELISA, Western Blotting (WB)

Détail du produit anti-POLR3A anticorps

Épitope: AA 1-200

Fonction: POLR3A antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène:

Immunogen sequence: 1-200aa

Immunogen: polymerase(RNA) III(DNA directed) polypeptide A, 155kDa

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur POLR3A

Antigène: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Autre désignation: POLR3A

Sujet:

Synonyms: DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1)|DNA-directed RNA polymerase III largest subunit|DNA-directed RNA polymerase III subunit A|RNA polymerase III 155 kDa subunit (RPC155)|RNA polymerase III subunit C160|POLR3A

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition(By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs(EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Poids moléculaire: 140-150 kDa

ID gène: 11128

UniProt: O14802