PUM2 anticorps

N° du produit ABIN7118125

Détail du produit anti-PUM2 anticorps

Antigène: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUM2 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: pumilio homolog 2(Drosophila)

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Date de péremption: 12 months

Détails sur PUM2

Antigène: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Autre désignation: PUM2 (PUM2 Produits)

Synonymes: anticorps pum-2, anticorps 5730503J23Rik, anticorps Pumm2, anticorps mKIAA0235, anticorps PUMH2, anticorps PUML2, anticorps pumilio RNA binding family member 2, anticorps pumilio RNA-binding family member 2, anticorps pum2, anticorps Pum2, anticorps PUM2

Sujet: Synonyms:KIAA0235, PUMH2 Background:Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element(PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element(NRE)(, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation(PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs(PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation(PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection(PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD(non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm(PubMed:26724866). May regulate DCUN1D3 mRNA levels(PubMed:25349211). May support proliferation and self-renewal of stem cells.

Poids moléculaire: 140 kDa

ID gène: 23369

UniProt: Q8TB72

Pathways: Ribonucleoprotein Complex Subunit Organization