USP10 anticorps

N° du produit ABIN7120491

Cet anticorps anti-USP10 est un anticorps Lapin Polyclonal détectant USP10 dans WB, ELISA et IF. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour USP10 anticorps (ABIN7120491)

Antigène: USP10 (Ubiquitin Specific Peptidase 10 (USP10))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp USP10 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-USP10 anticorps

Fonction: USP10 antibody

Purification: Immunogen affinity purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: ubiquitin specific peptidase 10

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:5000, IP: 1:200-1:2000, IF: 1:10-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months

Date de péremption: 12 months

Détails sur USP10

Antigène: USP10 (Ubiquitin Specific Peptidase 10 (USP10))

Autre désignation: USP10

Sujet:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 10|Deubiquitinating enzyme 10|Ubiquitin thioesterase 10|Ubiquitin-specific-processing protease 10|USP10|KIAA0190

Background: Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimulation or upon DNA damage(PubMed:25861989).

Poids moléculaire: 100 kDa

ID gène: 9100

UniProt: Q14694