TDRKH anticorps

N° du produit ABIN7504177

L’anticorps Souris Monoclonal anti-TDRKH a été validé pour WB, IF, IP, FACS et IHC (f). Il convient pour détecter TDRKH dans des échantillons de Humain.

Aperçu rapide pour TDRKH anticorps (ABIN7504177)

Antigène: TDRKH (Tudor and KH Domain Containing (TDRKH))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TDRKH est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-TDRKH anticorps

Specificité: Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

Réactivité croisée (Details): Human. Predicted to react with Mouse and Rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human TDRKH protein

Isotype: IgG2b

Information d'application

Indications d'application:

Positive Control: HeLa or MCF7 cells. Human testis or brain.

Known Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur TDRKH

Antigène: TDRKH (Tudor and KH Domain Containing (TDRKH))

Autre désignation: TDRKH

Sujet: 2700091C21Rik, Putative RNA binding protein, RGD1311196, RP11-98D18.8, TDRD2, TDRD7, Tdrkh, Tudor and KH domain containing, Tudor and KH domain-containing protein, tudor domain containing 2,TDRKH
Cellular localisation: Cytoplasm. Localized to cytosol and centrosome.

Poids moléculaire: 62.05kDa

ID gène: 11022, 144439

UniProt: Q9Y2W6