Recombinant NeuN anticorps (AA 30-60)
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- Antigène Voir toutes NeuN (RBFOX3) Anticorps
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
- Type d'anticorp
- Recombinant Antibody
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Épitope
- AA 30-60
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp NeuN est non-conjugé
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function, however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- A synthetic peptide corresponding to residues within aa 30-60 of human NeuN protein (exact sequence is proprietary)
- Isotype
- IgG
- Top Product
- Discover our top product RBFOX3 Anticorps primaire
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- Indications d'application
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Brain tissue.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
- Autre désignation
- RBFOX3 (RBFOX3 Produits)
- Synonymes
- anticorps FOX-3, anticorps FOX3, anticorps HRNBP3, anticorps NEUN, anticorps Fox-3, anticorps Hrnbp3, anticorps NeuN, anticorps Neuna60, anticorps Neun, anticorps RGD1560070, anticorps RNA binding fox-1 homolog 3, anticorps RNA binding protein, fox-1 homolog (C. elegans) 3, anticorps RNA binding protein, fox-1 homolog 3, anticorps RBFOX3, anticorps Rbfox3
- Sujet
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FLJ56884, FLJ58356, fox1 homolog C, Fox3, FOX3NeuN, hexaribonucleotide binding protein 3 (HRNBP3), neuronal nuclei, Rbfox3, RNA binding protein, fox 1 homolog (C. elegans) 3,Neuronal-Nuclei (NeuN) (Neuronal Marker)
Cellular localisation: Nucleus. Cytoplasm. - Poids moléculaire
- 38kDa
- ID gène
- 146713, 135229
- UniProt
- A6NFN3
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