Crossover junction endonuclease EME1 (EME1) anticorps

N° du produit ABIN7126263

L’anticorps anti-Crossover junction endonuclease EME1 Monoclonal Souris est utilisé pour la détection de Crossover junction endonuclease EME1 dans des échantillons de Humain. Il a été validé pour WB, IF et IHC.

Aperçu rapide pour Crossover junction endonuclease EME1 (EME1) anticorps (ABIN7126263)

Antigène: Crossover junction endonuclease EME1 (EME1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: MTA31 7H2-1

Détail du produit

Fonction: EME1 Antibody

Specificité: Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3' DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: His-tagged recombinant Eme1 of human origin

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293T or U-87 MG cells. Human tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Crossover junction endonuclease EME1 (EME1)

Autre désignation: EME1

Sujet:

Crossover junction endonuclease EME1, Essential Meiotic Endonuclease 1 Homolog 1/2, hMMS4, MMS4L, SLX2A

HEK293T or U-87 MG cells. Human tonsil.

Poids moléculaire: 65kDa

ID gène: 146956, 514330

UniProt: Q96AY2