Recombinant ERG anticorps (C-Term)

N° du produit ABIN7126343

Cet anticorps Lapin Monoclonal détecte spécifiquement ERG dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Souris, Cobaye et Hamster.

Aperçu rapide pour Recombinant ERG anticorps (C-Term) (ABIN7126343)

Antigène: ERG (ERG, ETS transcription factor (ERG))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Cobaye, Hamster

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp ERG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ERG-22R

Détail du produit anti-ERG anticorps

Épitope: C-Term

Fonction: Recombinant ERG / ETS Transcription Factor (Prostate Cancer Marker) Antibody

Specificité: ERG (ETS-related gene) is a proto-oncogene, a member of the ETS family of transcription factors. The ERG gene encodes for a nuclear protein, also called ERG, which is involved in hematopoietic and endothelial development. ERG remains constitually expressed in endothelial cells in blood and lymphatic vessels, and in bone marrow stem cells. ERG is expressed in virtually all endothelial neoplasms including hemangioendothelioma, angiosarcoma and Kaposi sarcoma. ERG is overexpressed secondary to gene rearrangement in cases of prostate adenocarcinoma, gastrointestinal stromal tumor, synovial sarcoma, meningioma, epithelioid sarcoma, malignant rhabdoid tumor, acute myeloid leukemia and blastic extramedullary myeloid tumor, and rarely Ewing sarcoma / primitive peripheral neuroectodermal tumor, chondrosarcoma, osteosarcoma, and rhabdomyosarcoma. For the identification of endothelial differentiation ERG seems more sensitive and specific than any other marker. Moreover, the interpretation is often easier due to the nuclear reaction, which also allows for double stains with cytoplasmic markers like podoplanin. Among carcinomas, ERG is highly specific for prostate, while the sensitivity is moderate.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Synthetic peptide corresponding to residues within aa450 to C-terminus of ERG was used as an immunogen

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, Ewing sarcoma or prostate adenocarcinoma tissues.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.Storage and StabilityAntibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur ERG

Antigène: ERG (ERG, ETS transcription factor (ERG))

Autre désignation: ERG

Sujet:

ERG, ERG/EWS fusion gene, ERG/FUS fusion gene, ERG/TMPSSR2 fusion gene, ERG1, ETS-related, KCNH2, Oncogene ERG, p55, TMPRSS2/ERG fusion

Ewing sarcoma or prostate adenocarcinoma tissues., Human tonsil, Human Lung, Skin, A431

Poids moléculaire: 55kDa

ID gène: 2078, 473819

UniProt: P11308