ESR2 anticorps (C-Term)

N° du produit ABIN7126353

L’anticorps Souris Monoclonal anti-ESR2 a été validé pour WB, IF, FACS et IHC (f). Il convient pour détecter ESR2 dans des échantillons de Humain.

Aperçu rapide pour ESR2 anticorps (C-Term) (ABIN7126353)

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ESR2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-ESR2 anticorps

Épitope: C-Term

Specificité: This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A synthetic peptide (CGMKMETLLPEATMEQ ) from the C terminus of the human estrogen receptor beta 2 isomer

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (2-4 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Human testis tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ESR2

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Autre désignation: ESR2

Sujet: Estrogen Receptor 2, Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2), Oestrogen Receptor Beta, Estrogen Receptor Beta, ER-BETA, ESTRB,ER-beta (Estrogen Receptor beta-2)
Cellular localisation: Nuclear

Poids moléculaire: 59kDa

ID gène: 2100, 660607

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling