FLI1 anticorps (AA 107-261)

N° du produit ABIN7126405

Cet anticorps anti-FLI1 est un anticorps Souris Monoclonal détectant FLI1 dans WB, IF et FACS. Adapté pour Humain.

Aperçu rapide pour FLI1 anticorps (AA 107-261) (ABIN7126405)

Antigène: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FLI1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-FLI1 anticorps

Épitope: AA 107-261

Specificité: Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing's Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (aa 107-261) of human FLI1 protein with hexa-histidine tag

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml for 60 minutes at RT);Optimal dilution for a specific application should be determined.

Positive_Control: THP1 and Raji cells. Tonsil, Ewing's sarcoma (EWS) and Angiosarcoma

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur FLI1

Antigène: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Autre désignation: FLI1

Sujet: ERGB transcription factor; Ewing Sarcoma breakpoint region 2 (EWSR2); FLI1; FLI1 EWS fusion gene; Friend leukemia integration 1 (FLI1) transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC1; Transcription factor ERGB; Viral integration region FLI1,FLI1 (Ewing's Sarcoma & Endothelial Marker)
Cellular localisation: Nuclear

Poids moléculaire: 51kDa

ID gène: 2313, 504281

UniProt: Q01543