GLUL anticorps (AA 50-250)

N° du produit ABIN7126480

Cet anticorps anti-GLUL est un anticorps Souris Monoclonal détectant GLUL dans WB et IHC (f). Adapté pour Humain.

Aperçu rapide pour GLUL anticorps (AA 50-250) (ABIN7126480)

Antigène: GLUL (Glutamate-Ammonia Ligase (GLUL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GLUL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-GLUL anticorps

Épitope: AA 50-250

Specificité: Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Réactivité croisée (Details): Human. Horse. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human brain, stomach or thyroid. A-431 or Y79 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur GLUL

Antigène: GLUL (Glutamate-Ammonia Ligase (GLUL))

Autre désignation: GLUL

Sujet: Cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS,Glutamine Synthetase / GLUL
Cellular localisation: Cytoplasm. Mitochondrion.

Poids moléculaire: 42kDa

ID gène: 2752, 518525

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion