GTF2A1 anticorps
Aperçu rapide pour GTF2A1 anticorps (ABIN7126495)
Antigène
Voir toutes GTF2A1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-i±, TFIIA-i² and TFIIA-i³, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.
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Réactivité croisée (Details)
- Human. Predicted to react in Mouse and Rat.
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Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogène
- Recombinant full-length human GTF2A1 protein
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Isotype
- IgG2b
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Indications d'application
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or MCF-7 cells.
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Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Agent conservateur
- Azide free
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Date de péremption
- 24 months
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- GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))
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Autre désignation
- GTF2A1
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Sujet
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General transcription factor IIA 1 19/37 kDa, General transcription factor IIA subunit 1, Glucose regulated protein 58kD pseudogene, TFIIA alpha p55, isoform 1, TFIIA p19 subunit, TFIIA p35 subunit, TFIIAL, Transcription initiation factor IIA beta chain, Transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit,GTF2A1/ TFIIA (Transcription Factor)
Cellular localisation: Nucleus -
Poids moléculaire
- 41.51kDa
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ID gène
- 2957, 592334
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UniProt
- P52655
Antigène
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