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GTF2A1 anticorps

Cet anticorps anti-GTF2A1 est un anticorps Souris Monoclonal détectant GTF2A1 dans ELISA, IP, IF, Coat et FACS. Adapté pour Humain.
N° du produit ABIN7126495

Aperçu rapide pour GTF2A1 anticorps (ABIN7126495)

Antigène

Voir toutes GTF2A1 Anticorps
GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

Reactivité

  • 38
  • 20
  • 12
  • 6
  • 6
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Humain

Hôte

  • 34
  • 4
Souris

Clonalité

  • 34
  • 4
Monoclonal

Conjugué

  • 26
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp GTF2A1 est non-conjugé

Application

  • 29
  • 13
  • 10
  • 5
  • 4
  • 4
  • 3
  • 1
ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS)
  • Specificité

    Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-i±, TFIIA-i² and TFIIA-i³, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.

    Réactivité croisée (Details)

    Human. Predicted to react in Mouse and Rat.

    Purification

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogène

    Recombinant full-length human GTF2A1 protein

    Isotype

    IgG2b
  • Indications d'application

    Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or MCF-7 cells.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Agent conservateur

    Azide free

    Stock

    -20 °C,-80 °C

    Stockage commentaire

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Date de péremption

    24 months
  • Antigène

    GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

    Autre désignation

    GTF2A1

    Sujet

    General transcription factor IIA 1 19/37 kDa, General transcription factor IIA subunit 1, Glucose regulated protein 58kD pseudogene, TFIIA alpha p55, isoform 1, TFIIA p19 subunit, TFIIA p35 subunit, TFIIAL, Transcription initiation factor IIA beta chain, Transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit,GTF2A1/ TFIIA (Transcription Factor)
    Cellular localisation: Nucleus

    Poids moléculaire

    41.51kDa

    ID gène

    2957, 592334

    UniProt

    P52655
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