PDX1 anticorps

N° du produit ABIN7126596

L’anticorps Souris Monoclonal anti-PDX1 a été validé pour IF et FACS. Il convient pour détecter PDX1 dans des échantillons de Humain.

Aperçu rapide pour PDX1 anticorps (ABIN7126596)

Antigène: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDX1 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-PDX1 anticorps

Specificité: Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic i²-cells. PDX-1 is a key regulator of pancreatic islet development and Insulin gene transcription in i²-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3i², HNF-1i± and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by Glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of i²-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.

Réactivité croisée (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human PDX1 protein

Isotype: IgG2

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HEK293 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur PDX1

Antigène: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Autre désignation: PDX1

Sujet: Glucose sensitive factor, GSF, IDX1, Insulin promoter factor 1 homeodomain transcription factor, IPF1, STF1, IUF1, Islet/duodenum homeobox 1, MODY4, Pancreas/duodenum homeobox, Somatostatin transactivating factor 1,Pancreatic duodenal homeobox-1 protein (PDX1)
Cellular localisation: Nucleus.

Poids moléculaire: 46kDa

ID gène: 3651, 32938

UniProt: P52945

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location