Keratin 3 anticorps

N° du produit ABIN7126611

L’anticorps Souris Monoclonal anti-Keratin 3 a été validé pour WB et IHC (f). Il convient pour détecter Keratin 3 dans des échantillons de Lapin.

Aperçu rapide pour Keratin 3 anticorps (ABIN7126611)

Antigène: Keratin 3 (KRT3)

Reactivité: Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Keratin 3 anticorps

Specificité: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins, which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy.KRT3/2542 recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Réactivité croisée (Details): Human, rabbit and bovine.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Human cornea.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Keratin 3

Antigène: Keratin 3 (KRT3)

Autre désignation: KRT3

Sujet: 65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3,Cytokeratin 3 (KRT3) (Corneal Epithelial Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 65kDa

ID gène: 38550, 680652

UniProt: P12035