Recombinant KRT14 anticorps (AA 350-472)

N° du produit ABIN7126632

L’anticorps anti-KRT14 Monoclonal Lapin est utilisé pour la détection de KRT14 dans des échantillons de Humain. Il a été validé pour IHC et WB.

Aperçu rapide pour Recombinant KRT14 anticorps (AA 350-472) (ABIN7126632)

Antigène: KRT14 (Keratin 14 (KRT14))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT14 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: KRT14-4584R

Détail du produit anti-KRT14 anticorps

Épitope: AA 350-472

Fonction: Recombinant Cytokeratin 14 (KRT14) (Squamous Cell Marker) Antibody

Specificité: Cytokeratin 14 is a member of the type I keratin family of intermediate filament proteins. It always pairs with the type II keratin K5 and form the primary keratin pair of the keratinocytes of stratified squamous epithelia, including the epidermis as well as mucosal non-keratinizing stratified squamous epithelia.Cytokeratin 14 is strongly expressed in the undifferentiated basal cell layer containing the stem cells and are down-regulated in the differentiating suprabasal cell layers. Otherwise, in the widely well stratified follicular outer root sheath, cytokeratin 14 is uniformly expressed throughout all layers. The expression spectrum of cytokeratin 14 in tumors corresponds well to the patterns in normal epithelia. Thus, most squamous cell carcinomas as well as malignant mesotheliomas strongly express this keratin whereas little, focal, or no expression is found in adenocarcinomas.Cytokeratin 14 may be a useful marker in the differential diagnosis of squamous cell carcinoma from other epithelial tumors. Recent studies also indicate that CK14 expression in breast cancer corresponded with poor clinical outcome and that CK14 may have diagnostic value in the sub-classification of NSCLC.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogène: Recombinant human KRT14 fragment (around aa350-472) (exact sequence is proprietary)

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431 orHeLacells. Human prostate, skin or squamous cell carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur KRT14

Antigène: KRT14 (Keratin 14 (KRT14))

Autre désignation: KRT14

Sujet:

CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, k14, Keratin 14, Keratin Type I Cytoskeletal 14, Koebner, krt14, NFJ

A-431 or HeLa cells. Human prostate, Skin or squamous cell carcinoma.

Poids moléculaire: ~50kDa

ID gène: 3861, 6545380

UniProt: P02533