MEF2D anticorps

N° du produit ABIN7126680

L’anticorps Souris Monoclonal anti-MEF2D a été validé pour FACS, IP, IF et IHC (f). Il convient pour détecter MEF2D dans des échantillons de Humain.

Aperçu rapide pour MEF2D anticorps (ABIN7126680)

Antigène: MEF2D (Myocyte Enhancer Factor 2D (MEF2D))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MEF2D est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-MEF2D anticorps

Specificité: The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF4, are one class of identified factors. A second family of DNA-binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.

Réactivité croisée (Details): Human. Predicted to react in Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human MEF2D protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MEF2D

Antigène: MEF2D (Myocyte Enhancer Factor 2D (MEF2D))

Autre désignation: MEF2D

Sujet: MADS box transcription factor 2 polypeptide D, Myocyte specific enhancer factor 2, polypeptide D,MEF2D / Myocyte enhancer factor 2D (Transcription Factor)
Cellular localisation: Nucleus. Translocated by HDAC4 to nuclear dots.

Poids moléculaire: 55.94kDa

ID gène: 4209, 314327

UniProt: Q14814