Recombinant CD99 anticorps (C-Term)

N° du produit ABIN7126690

Cet anticorps anti-CD99 Monoclonal Lapin (Clone MIC2-3478R) (ABIN7126690) détecte spécifiquement CD99 dans IHC et WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant CD99 anticorps (C-Term) (ABIN7126690)

Antigène: CD99

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD99 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: MIC2-3478R

Détail du produit anti-CD99 anticorps

Épitope: C-Term

Fonction: Recombinant CD99 / MIC2 (Ewing s Sarcoma Marker) Antibody

Specificité: CD99 is a type I transmembrane glycoprotein encoded by the CD99 gene and the functions of CD99 in cells in which CD99 was highly expressed have been studied and they were as follows: cell death of thymocytes and T lymphocytes, migration through monocyte endothelial junctions by adhesion and diapedesis, cell-cell adhesion in lymphocytes, maintenance of cellular morphology in Hodgkin and Reed/Sternberg cells, and recruitment of T cells.CD99 expression has been reported in many cell types, such as hematopoietic cells, endothelial cells, central nervous system ependymal cells, thymocytes, granular cells of the ovary, Sertoli cells, and pancreatic islet cells. And in tumors it expressed by virtually almost all Ewing s sarcoma and primitive peripheral neuroectodermal tumors (ES/PNET) and demonstrates strong and diffuse membranous staining. Other tumors that may show CD99 expression include neuroendocrine carcinomas, mesenchymal chondrosarcomas, solitary fibrous tumors, synovial sarcomas, vascular tumors, small round blue cell tumors, lymphoblastic lymphoma, acute myeloid leukemia, and myeloid sarcoma. Studies have shown that CD99 may be a sensitive marker for Ewing s sarcoma and peripheral neuroectodermal tumors.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified by Protein A Column.

Immunogène: Synthetic peptide corresponding to CD99 residues within aa85 C terminal of CD99 was used as an immunogen

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4 cells. Pancreas or Ewing s sarcoma

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD99

Antigène: CD99

Autre désignation: CD99

Sujet:

12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2

MCF7, A549, MOLT-4 cells. Pancreas or Ewing s sarcoma

Poids moléculaire: 27-32kDa

ID gène: 4267, 653349

UniProt: P14209