Recombinant Ki-67 anticorps (AA 2293-2478)

N° du produit ABIN7126698

L’anticorps anti-Ki-67 Monoclonal Lapin est utilisé pour la détection de Ki-67 dans des échantillons de Humain. Il a été validé pour IHC et WB.

Aperçu rapide pour Recombinant Ki-67 anticorps (AA 2293-2478) (ABIN7126698)

Antigène: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Ki-67 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: MKI67-4945R

Détail du produit anti-Ki-67 anticorps

Épitope: AA 2293-2478

Fonction: Recombinant Ki-67 (Proliferating Cell Marker) Antibody

Specificité: Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Any actively proliferating cells. Human skin, tonsil or lymph node.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Ki-67

Antigène: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Autre désignation: MKI67

Sujet:

KI-67, Ki67, KI-67 Antigen (KIA), MKI67, Proliferation related Ki-67 antigen

Any actively proliferating cells. Human skin, tonsil or lymph node. Raji, Ramos

Poids moléculaire: 345kDa and 395kDa

ID gène: 4288, 689823

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process