Recombinant MUC1 anticorps

N° du produit ABIN7126720

Cet anticorps anti-MUC1 est un anticorps Souris Monoclonal détectant MUC1 dans WB et IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant MUC1 anticorps (ABIN7126720)

Antigène: MUC1 (Mucin 1 (MUC1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-MUC1 anticorps

Specificité: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Réactivité croisée (Details): Human and Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human MUC1 protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: MUC1

Sujet: Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen,MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)
Cellular localisation: Cytoplasmic and cell surface

Poids moléculaire: 265-400kDa

ID gène: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling