Recombinant MUC3A anticorps

N° du produit ABIN7126724

Cet anticorps Souris Monoclonal détecte spécifiquement MUC3A dans IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant MUC3A anticorps (ABIN7126724)

Antigène: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC3A est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: RMUC3-1154

Détail du produit anti-MUC3A anticorps

Fonction: Recombinant MUC3 (Mucin 3) Antibody

Specificité: It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues.The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human MUC3A protein (exact sequence is proprietary)

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: LS174T cells.Human colon or colon carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MUC3A

Antigène: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Autre désignation: MUC3A

Sujet:

Intestinal mucin-3A, intestinal mucin-like, MUC3, MUC3A, MUC3B, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin 3B, cell surface associated

LS174T cells.Human colon or colon carcinoma.

Poids moléculaire: 1100kDa

ID gène: 4584, 57876

UniProt: Q02505