Myogenin anticorps

N° du produit ABIN7126738

Cet anticorps anti-Myogenin est un anticorps Souris Monoclonal détectant Myogenin dans WB, ELISA, IF, FACS, IP et Coat. Adapté pour Humain.

Aperçu rapide pour Myogenin anticorps (ABIN7126738)

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Myogenin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Coating (Coat)

Détail du produit anti-Myogenin anticorps

Specificité: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80 % amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Réactivité croisée (Details): Human, Mouse andRat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Human myogenin recombinant protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Rh-30 or HeLa cells. Human skeletal muscle or rhabdomyosarcoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Myogenin

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Autre désignation: MYOG

Sujet: BHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4,Myogenin / Myf-4 (Skeletal Muscle Marker) (Transcription Factor)
Cellular localisation: Nucleus.

Poids moléculaire: 34kDa

ID gène: 4656, 2830

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development