Neurogenin 3 anticorps

N° du produit ABIN7126754

Cet anticorps Souris Monoclonal détecte spécifiquement Neurogenin 3 dans ELISA, IP, FACS et Coat. Il présente une réactivité envers Humain.

Aperçu rapide pour Neurogenin 3 anticorps (ABIN7126754)

Antigène: Neurogenin 3 (NEUROG3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Neurogenin 3 est non-conjugé

Application: ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Détail du produit anti-Neurogenin 3 anticorps

Specificité: This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human full-lengthNEUROG3protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human fetal gut, colon, liver or heart.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Neurogenin 3

Antigène: Neurogenin 3 (NEUROG3)

Autre désignation: NEUROG3

Sujet: ATH4B, Atoh5, bHLHa7, Class A basic helix-loop-helix protein 7, Math4B, NEUROG3, Neurogenin-3, Neurogenin3, NGN-3, ngn3, Protein atonal homolog 5,NEUROG3 / Neurogenin 3 / NGN3 (Transcription Factor)
Cellular localisation: Nucleus.

Poids moléculaire: 23.54kDa

ID gène: 50674, 288655

UniProt: Q9Y4Z2