TDP2 anticorps
-
- Antigène Voir toutes TDP2 Anticorps
- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp TDP2 est non-conjugé
-
Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- This MAb recognizes a protein of 41kDa, which is identified as TDP2. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant full-length human TDP2 protein
- Isotype
- IgG2b
- Top Product
- Discover our top product TDP2 Anticorps primaire
-
-
- Indications d'application
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Lung, Duodenum or Small Intestinal Carcinoma.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
-
- Antigène
- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
- Autre désignation
- TDP2 (TDP2 Produits)
- Synonymes
- anticorps AD022, anticorps EAP2, anticorps EAPII, anticorps TTRAP, anticorps dJ30M3.3, anticorps hTDP2, anticorps ad022, anticorps eap2, anticorps ttrap, anticorps D13Ertd656e, anticorps Ttrap, anticorps tdp2, anticorps ttrapl, anticorps tyrosyl-DNA phosphodiesterase 2, anticorps tyrosyl-DNA phosphodiesterase 2 S homeolog, anticorps tyrosyl-DNA phosphodiesterase 2b, anticorps TDP2, anticorps tdp2.S, anticorps Tdp2, anticorps tdp2b
- Sujet
-
5'-Tyr-DNA phosphodiesterase, EAP2, EAPII, ETS1-associated protein 2, ETS1-associated protein II, TRAF and TNF receptor-associated protein, TTRAP, Tyr-DNA phosphodiesterase 2, Tyrosyl-DNA phosphodiesterase 2 (TDP2),TDP2 / EAPII
Cellular localisation: Nuclear and Cytoplasmic - Poids moléculaire
- 41kDa
- ID gène
- 51567, 728795
- UniProt
- O95551
-