Recombinant KRT20 anticorps (AA 196-323)
Aperçu rapide pour Recombinant KRT20 anticorps (AA 196-323) (ABIN7126808)
Antigène
Voir toutes KRT20 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 196-323
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Specificité
- This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
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Réactivité croisée (Details)
- Human.
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Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogène
- Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
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Isotype
- IgG2a
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Indications d'application
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HT29 cells. Human normal colon or colon adenocarcinoma.
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Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Agent conservateur
- Azide free
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Date de péremption
- 24 months
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- KRT20 (Keratin 20 (KRT20))
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Autre désignation
- KRT20
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Sujet
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CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)
Cellular localisation: Cytoplasm. -
Poids moléculaire
- 46kDa
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ID gène
- 54474, 84905
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UniProt
- P35900
Antigène
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