PRMT6 anticorps

N° du produit ABIN7126821

L’anticorps Souris Monoclonal anti-PRMT6 a été validé pour IF, IP et FACS. Il convient pour détecter PRMT6 dans des échantillons de Humain.

Aperçu rapide pour PRMT6 anticorps (ABIN7126821)

Antigène: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PRMT6 est non-conjugé

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Détail du produit anti-PRMT6 anticorps

Specificité: A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMTs contain an S-adenosylmethione motif which functions to add one or two methyl groups to guanidino nitrogens of arginine (R) side chains. PRMT6, also known as HRMT1L6, is a nuclear protein belonging to the PRMT family and is predominantly expressed in testis and kidney. It is known to methylate Histones H3, H4 and H2A. PRMT6 is the major dimethyltransferase for Histone H3 and specifically methylates Histone H3 at R2. Methylation at Histone H3 R2 acts to inhibit Histone H3 K4 trimethylation and ultimately leads to the transcriptional repression of genes that are activated by Histone H3 K4 trimethylation. In addition, PRMT6 methylates HIV TAT, possibly functioning as a form of cellular innate immunity to restrict levels of HIV replication.

Réactivité croisée (Details): Human. Predicted to react with Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human PRMT6 protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF7 or HepG2 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur PRMT6

Antigène: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Autre désignation: PRMT6

Sujet: Chromobox protein homolog 7, Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6, Histone-arginine N-methyltransferase PRMT6, HMT1 hnRNP methyltransferase like 6., HRMT1L6,PRMT6
Cellular localisation: Nucleus.

Poids moléculaire: 42kDa

ID gène: 55170, 26006

UniProt: Q96LA8