SP100 anticorps

N° du produit ABIN7126965

Cet anticorps Souris Monoclonal détecte spécifiquement SP100 dans WB, ELISA, IP, IF, Coat, FACS et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour SP100 anticorps (ABIN7126965)

Antigène: SP100 (SP100 Nuclear Antigen (SP100))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SP100 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-SP100 anticorps

Specificité: The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human SP100 protein

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Immunofluorescence (0.5-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Flow Cytometry (0.5-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur SP100

Antigène: SP100 (SP100 Nuclear Antigen (SP100))

Autre désignation: SP100

Sujet: Lysp100b, Nuclear antigen Sp100, Nuclear autoantigen Sp 100, Nuclear dot associated Sp100 protein, SP100, SP100 HMG nuclear autoantigen, SP100 nuclear antigen, Speckled 100 kDa,SP100
Cellular localisation: Nucleus. PML body.

Poids moléculaire: 100.4kDa

ID gène: 6672, 369056

UniProt: P23497

Pathways: Retinoic Acid Receptor Signaling Pathway