Recombinant TIA1 anticorps

N° du produit ABIN7127002

L’anticorps Lapin Monoclonal anti-TIA1 a été validé pour IHC (f). Il convient pour détecter TIA1 dans des échantillons de Humain.

Aperçu rapide pour Recombinant TIA1 anticorps (ABIN7127002)

Antigène: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp TIA1 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-TIA1 anticorps

Specificité: The T cell intracellular antigen 1 (TIA 1) is a 17 kDa cytoplasmic granule associated protein also designated as GMP 17, for granule membrane protein of 17 kDa. The GMP 17/TIA 1 Molecule is expressed in cells possessing cytolytic potential and could be involved in the signaling cascade of Fas (CD95) mediated apoptosis. Within hematopoietic cell lines, the TIA1/1352R monoclonal antibody reacts with about 90 % of CD16+, 50 60 % of CD8+, and less than 10 % of CD4+ normal peripheral blood lymphocytes. It reacts with almost all monocytes and granulocytes. This antibody also reacts with CD4+ activated T cell clones, activated NK cell clones, and Con activated thymocytes, but not with B lymphocytes or B cell lines. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Human bone marrow malignant cells from a non B, non T acute leukemia

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, spleen and lymph node.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur TIA1

Antigène: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Autre désignation: TIA1

Sujet: Cytotoxic granule associated RNA binding protein 1, mTIA-1, Nucleolysin TIA1 isoform p40, p40-TIA-1, RNA binding protein TIA1, TIAL1, TIAR, WDM,TIA1 (T-Cell-Restricted Intracellular Antigen-1)
Cellular localisation: Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.

Poids moléculaire: 40kDa

ID gène: 7072, 413123

UniProt: P31483