Recombinant Vimentin anticorps (AA 2-466)

N° du produit ABIN7127048

Cet anticorps anti-Vimentin Monoclonal Souris (Clone rVIM-6431) (ABIN7127048) détecte spécifiquement Vimentin dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Rat, Porc, Boeuf (Vache), Poulet, Chien, Cat et Cheval.

Aperçu rapide pour Recombinant Vimentin anticorps (AA 2-466) (ABIN7127048)

Antigène: Vimentin (VIM)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Porc, Boeuf (Vache), Poulet, Chien, Cat, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RVIM-6431

Détail du produit anti-Vimentin anticorps

Épitope: AA 2-466

Fonction: Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

Specificité: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Réactivité croisée (Details): Human, Rat, Horse, Chicken, Cow, Cat, Dog, and Pig. Very broad species reactivity. Does not react with Mouse.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogène: A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U-87, Raji, Jurkat or HeLa cells. Human lymph node or tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: VIM

Sujet:

VIM

Raji, U-87, Jurkat or HeLa cells. Human lymph node or tonsil. Human Heart, Human Skeletal muscle, Human Skin, A549.

Poids moléculaire: 57-60kDa

ID gène: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis