Recombinant Vimentin anticorps (AA 2-466)

N° du produit ABIN7127051

Cet anticorps anti-Vimentin est un anticorps Lapin Monoclonal détectant Vimentin dans IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant Vimentin anticorps (AA 2-466) (ABIN7127051)

Antigène: Vimentin (VIM)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-Vimentin anticorps

Épitope: AA 2-466

Specificité: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Réactivité croisée (Details): Human, Rat, Horse, Chicken, Cow, Cat, Dog, and Pig. Very broad species reactivity. Does not react with Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U-87, Raji, Jurkat or HeLa cells. Human lymph node or tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: VIM

Sujet: VIM,Vimentin (Mesenchymal Cell Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 57-60kDa

ID gène: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis