Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Recombinant WT1 anticorps (AA 1-100)

WT1 Reactivité: Humain IHC (f) Hôte: Lapin Monoclonal unconjugated Recombinant Antibody
N° du produit ABIN7127057
  • Antigène Voir toutes WT1 Anticorps
    WT1 (Wilms Tumor 1 (WT1))
    Type d'anticorp
    Recombinant Antibody
    Épitope
    • 32
    • 16
    • 15
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-100
    Reactivité
    • 144
    • 67
    • 60
    • 17
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 81
    • 62
    • 1
    Lapin
    Clonalité
    • 85
    • 59
    Monoclonal
    Conjugué
    • 70
    • 10
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp WT1 est non-conjugé
    Application
    • 87
    • 81
    • 61
    • 56
    • 32
    • 21
    • 20
    • 14
    • 13
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificité
    The WT1 gene located at chromosome 11p13 codes for a transcription factor, a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62 kDa produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an inactive form. When activated through phosphorylation it is translocated to the nucleus. WT1 influences cell proliferation by suppressing bcl-2 and regulating cadherin and p53. In normal epithelia, nuclear WT1 expression is largely restricted to ovary (surface epithelium and inclusion cysts) and fallopian tube, while WT1 is not found in endometrial or cervical epithelium. As regards nonepithelial cells, nuclear WT1 is found in mesothelium and some sub mesothelial stromal cells, stromal cells of the female genital tract, testicular non-germinal cells, and kidney (podocytes). In tumor tissues, WT1 is detected in tumor cells of Wilms Tumor (also known as nephroblastoma) and mesothelioma. Additionally, WT1 expression has been found in ovarian serous carcinomas and some breast carcinomas.WT1 is particularly used for distinguishing malignant mesothelioma and ovarian serous carcinoma from nonserous carcinomas. As for malignant mesothelioma, calretinin and WT1 are superior to cytokeratin 5/6, N-cadherin and thrombomodulin. WT1 is also applicable for the differential diagnostic of small cell childhood tumors.
    Réactivité croisée (Details)
    Human.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogène
    Recombinant fragment (around aa1-100) of human WT1 protein (exact sequence is proprietary)
    Isotype
    IgG
    Top Product
    Discover our top product WT1 Anticorps primaire
  • Indications d'application

    Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: K562 cells. Human Wilm's tumor, mesothelioma or fetal kidney.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Agent conservateur
    Azide free
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Date de péremption
    24 months
  • Antigène
    WT1 (Wilms Tumor 1 (WT1))
    Autre désignation
    WT1 (WT1 Produits)
    Synonymes
    anticorps AWT1, anticorps EWS-WT1, anticorps GUD, anticorps NPHS4, anticorps WAGR, anticorps WIT-2, anticorps WT33, anticorps CWT1, anticorps WT1, anticorps XWT1a, anticorps wt-1, anticorps wt1, anticorps xWT1, anticorps WT-1, anticorps gud, anticorps wagr, anticorps wt33, anticorps xwt1, anticorps wit-2, anticorps si:ch211-160j6.3, anticorps zgc:152697, anticorps D630046I19Rik, anticorps Wt-1, anticorps XWT1b, anticorps XeWT1, anticorps Wilms tumor 1, anticorps Wilms tumor 1 S homeolog, anticorps wilms tumor 1b, anticorps Wilms tumor protein, anticorps Wilms tumor 1 homolog, anticorps Wilms tumor 1 L homeolog, anticorps WT1, anticorps wt1.S, anticorps wt1, anticorps wt1b, anticorps Wt1, anticorps wt1.L
    Sujet
    WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1,Wilm\'s Tumor 1 (WT1) (Wilm\'s Tumor & Mesothelial Marker)
    Cellular localisation: Nucleus and cytoplasm. Shuttles between nucleus and cytoplasm.
    Poids moléculaire
    49kDa
    ID gène
    7490, 591980
    UniProt
    P19544
    Pathways
    Tube Formation
Vous êtes ici:
Support technique