Recombinant p63 anticorps (AA 1-100)

N° du produit ABIN7127111

Cet anticorps anti-p63 est un anticorps Lapin Monoclonal détectant p63 dans ELISA, FACS, IHC (f) et Coat. Adapté pour Humain.

Aperçu rapide pour Recombinant p63 anticorps (AA 1-100) (ABIN7127111)

Antigène: p63 (TP63) (Tumor Protein P63 (TP63))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp p63 est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Détail du produit anti-p63 anticorps

Épitope: AA 1-100

Specificité: P40 antibody (antibody recognizing Np63 only) is an isoform of p63, is thought to function as a stem cell factor, responsible for maintaining cells in an uncommitted state with regenerative potential a role that may be recapitulated in tumors derived from these cells. p40 is normally expressed in the basal or progenitor cell layer of stratified epithelia (eg. squamous, urothelial, bronchial), basal cells of some glandular epithelia (eg. prostate), as well as myoepithelial cells of breast and salivary glands, trophoblasts and thymic epithelial cells. In tumor tissues, p40 expression is specific for squamous cell carcinoma. p40 is equivalent to p63 in sensitivity for lung squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. p40 appears to be a more reliable marker for squamous cell carcinoma.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa1-100) of human p40 protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293, MDA-MB453 or MCF7 cells. Human prostate, lung or bladder squamous cell carcinoma tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur p63

Antigène: p63 (TP63) (Tumor Protein P63 (TP63))

Autre désignation: TP63

Sujet: Amplified in squamous cell carcinoma (AIS), Chronic ulcerative stomatitis protein (CUSP), EEC3, TP73L, LMS, TP63, Keratinocyte transcription factor KET, NBP, P51/P63, p53 like transcription factor, RHS, SHFM4, TAp63alpha, TP53CP, TP53L, Transformation-related protein 63, Trp53rp1, Trp6,3, Tumor protein p53-like, tumor protein p73-like,p40 (Squamous, Basal & Myoepithelial Cell Marker)
Cellular localisation: Nucleus.

Poids moléculaire: 63kDa

ID gène: 8626, 137569

UniProt: Q9H3D4