CD151 anticorps

N° du produit ABIN7127232

Cet anticorps Souris Monoclonal détecte spécifiquement CD151 dans WB, FACS, IF et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour CD151 anticorps (ABIN7127232)

Antigène: CD151

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD151 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-CD151 anticorps

Specificité: CD151 is involved in a wide variety of cell biological processes, including cell adhesion and the transport of integrins via vesicles. The human CD151 gene maps to chromosome 11p15.5 and encodes a 253 amino acid protein, which belongs to the tetraspan (4TM) superfamily. CD151 can associate with several integrin chains including 1, 3, 4, i±2, i±3, i±5 and i±6 integrins. CD151 may provide a framework for the spatial organization of both type I and type II hemidesmosomes, which are specialized junctional complexes that function as cell attachment sites for binding to basement membranes. CD151 RNA transcript (1.6 kb) can be detected in MO7e cells, bone marrow stromal cells, C11 endothelial cells, HUVEC and several myeloid leukemia cell lines, however, no transcript is detected in brain and the lymphoblastoid cell lines MOLT-4 and BALM-1. Leu 149-Glu 213 of CD151 is the interface through which Integrin i±3/ 1 can bind. CD151 can enhance cell motility, invasion and metastasis of cancer cells in a focal adhesion kinase dependent manner.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: The details of the immunogen for this antibody are not available.

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil or spleen. HT1080 cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD151

Antigène: CD151

Autre désignation: CD151

Sujet: GP27, hemidesmosomal tetraspanin CD151, Membrane glycoprotein SFA-1, MER2, Platelet endothelial tetraspan antigen 3 (PETA3), platelet surface glycoprotein gp27, Platelet-endothelial cell tetraspan antigen 3, Platelet-endothelial tetraspan antigen 3, RAPH, Red blood cell antigen MER2, SFA1, Tetraspanin-24 (TSPAN24),CD151
Cellular localisation: Cell Surface

Poids moléculaire: 28-32kDa

ID gène: 977, 654379

UniProt: P48509