KRT10, KRT13 anticorps

N° du produit ABIN7127244

Cet anticorps Souris Monoclonal détecte spécifiquement KRT10, KRT13 dans FACS, IF, IHC et WB. Il présente une réactivité avec des échantillons de Cat et Humain.

Aperçu rapide pour KRT10, KRT13 anticorps (ABIN7127244)

Antigène: KRT10, KRT13

Reactivité: Cat, Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: DE-K13

Détail du produit

Fonction: Cytokeratin 10/13 Antibody

Specificité: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Réactivité croisée (Details): Human and Cat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Cytoskeletal preparation extracted from human ectocervical epithelium

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: A431, HeLa or MCF7 cells. Human tonsil, esophageal or bladder carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: KRT10, KRT13

Autre désignation: KRT10 & KRT13

Sujet:

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT13, Type I keratin Ka13

A431, esophageal or bladder carcinoma., HeLa or MCF7 cells. Human tonsil

Poids moléculaire: 56.5kDa (CK10) & 53kDa (CK13)