KRT5 & KRT6 anticorps

N° du produit ABIN7127253

Cet anticorps Souris Monoclonal détecte spécifiquement KRT5 & KRT6 dans IHC (f). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour KRT5 & KRT6 anticorps (ABIN7127253)

Antigène: KRT5 & KRT6

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit

Specificité: It recognizes polypeptides of 58 kDa and 56 kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma. It does not react with pulmonary adenocarcinoma.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human KRT5 protein

Isotype: IgG1, IgG2a

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or skin tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détail du antigène

Antigène: KRT5 & KRT6

Sujet: CK5, Keratin-5, KRT5, Cytoskeletal 6A, KRT6A,Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 58kDa (KRT5), 56kDa (KRT6A)