Recombinant DGCR8 anticorps

N° du produit ABIN7127453

Cet anticorps Lapin Monoclonal détecte spécifiquement DGCR8 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant DGCR8 anticorps (ABIN7127453)

Antigène: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp DGCR8 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 2H2

Détail du produit anti-DGCR8 anticorps

Fonction: DGCR8 Recombinant Monoclonal Antibody

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human DGCR8

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:5000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur DGCR8

Antigène: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Autre désignation: DGCR8

Sujet:

Background: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).

Aliases: Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12, DGCRK6, LP4941

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways