POLR2A/RPB1 anticorps (AA 76-298)

N° du produit ABIN7150422

L’anticorps Lapin Polyclonal anti-POLR2A/RPB1 a été validé pour IHC, IF et ELISA. Il convient pour détecter POLR2A/RPB1 dans des échantillons de Humain.

Aperçu rapide pour POLR2A/RPB1 anticorps (AA 76-298) (ABIN7150422)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Détail du produit anti-POLR2A/RPB1 anticorps

Épitope: AA 76-298

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human DNA-directed RNA polymerase II subunit RPB1 protein (76-298AA)

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: IHC:1:200-1:500, IF:1:100-1:500,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: POLR2A

Sujet:

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity).

Aliases: DNA directed RNA polymerase II A antibody, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody, DNA-directed RNA polymerase II subunit A antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, DNA-directed RNA polymerase III largest subunit antibody, hRPB220 antibody, hsRPB1 antibody, POLR2 antibody, Polr2a antibody, POLRA antibody, Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa antibody, Polymerase (RNA) II (DNA directed) polypeptide A antibody, RNA polymerase II subunit B1 antibody, RNA-directed RNA polymerase II subunit RPB1 antibody, RPB1 antibody, RPB1_HUMAN antibody, RPBh1 antibody, RpIILS antibody, RPO2 antibody, RPOL2 antibody

UniProt: P24928

Pathways: Regulatory RNA Pathways