SdeA (AA 19-268) anticorps (FITC)

N° du produit ABIN7176792

L’anticorps anti-SdeA Polyclonal Lapin est utilisé pour la détection de SdeA dans des échantillons de Legionella pneumophila.

Aperçu rapide pour SdeA (AA 19-268) anticorps (FITC) (ABIN7176792)

Antigène: SdeA

Reactivité: Legionella pneumophila

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: FITC

Application: Veuillez nous consulter SVP

Détail du produit

Épitope: AA 19-268

Fonction: sdeA Antibody, FITC conjugated

Réactivité croisée: Legionella pneumophila

Purification: Protein G purified

Pureté: >95 %

Immunogène: Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268AA)

Isotype: IgG

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détail du antigène

Antigène: SdeA

Sujet:

Background: Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2). Transfers an ADP-ribose moiety from NAD to the \'Arg-42\' residue of ubiquitin in a reaction that releases nicotinamide. The modified ubiquitin is subsequently transferred to the substrate protein through an unknown mechanism that results in the release of AMP. Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PubMed:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains (\'Lys-11\', \'Lys-48\' and \'Lys-63\') with a distinct preference for \'Lys-63\' linkages, is thus able to efficiently remove \'Lys-63\'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication (PubMed:26598703).

Aliases: sdeA antibody, lpg2157 antibody, Ubiquitinating/deubiquitinating enzyme SdeA antibody, Effector protein SdeA) [Includes: Deubiquitinase antibody, DUB antibody, EC 3.4.22.- antibody, Deneddylase antibody, Deubiquitinating enzyme), Ubiquitin transferase antibody, EC 2.3.2.-), Mono-ADP-ribosyltransferase antibody, mART antibody, EC 2.4.2.31)] antibody

UniProt: Q5ZTK4