Tumor Suppressor ARF (CDKN2A) (AA 1-156) anticorps

N° du produit ABIN7193535

Cet anticorps anti-Tumor Suppressor ARF Monoclonal Souris (Clone 4A10E1) (ABIN7193535) détecte spécifiquement Tumor Suppressor ARF dans ELISA, FACS et ICC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Tumor Suppressor ARF (CDKN2A) (AA 1-156) anticorps (ABIN7193535)

Antigène: Tumor Suppressor ARF (CDKN2A)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 4A10E1

Détail du produit

Épitope: AA 1-156

Fonction: CDKN2A/P16 Antibody

Purification: Purified antibody

Immunogène: Purified recombinant fragment of human CDKN2A/P16 (AA: 1-156) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/500 - 1/2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Détail du antigène

Antigène: Tumor Suppressor ARF (CDKN2A)

Autre désignation: CDKN2A/P16

Sujet:

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene, this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Poids moléculaire: 16.5 kDa

UniProt: P42771