CDH6 anticorps (AA 281-380)

N° du produit ABIN715286

L’anticorps Lapin Polyclonal anti-CDH6 a été validé pour WB, ELISA et FACS et a été validé indépendamment. Il convient pour détecter CDH6 dans des échantillons de Humain.

Aperçu rapide pour CDH6 anticorps (AA 281-380) (ABIN715286)

Antigène: CDH6 (K-Cadherin (CDH6))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDH6 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-CDH6 anticorps

Épitope: AA 281-380

Réactivité croisée: Humain

Homologie: Mouse,Rat,Cow,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human K Cadherin

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029743 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029743

Date: 01.07.2014

Numéro du lot: 130823

Application validée: Flow Cytometry

Contrôle positif: 786-O cells and [MCF-7 cells] (http://www.nature.com/bjc/journal/v90/n5/fig_tab/6601640f10.html#figure-title)

Conclusion: As expected, strong signal was observed in the cells stained with anti-K-Cadherin (CDH6) antibody. A small amount of non-specific staining was observed from the isotype and secondary antibody only negative controls compared with unstained cells.

Validation Images

Figure 1. Histogram of 786-O cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Figure 2. Histogram of MCF-7 cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Protocole

Anticorps primaire:

• Antigen: K-Cadherin (CDH6) antibody
• Catalog number: ABIN715286
• Lot number: 130823

Anticorps secondaire:

• Antibody: Goat anti-rabbit IgG-Alexa 647

Full Protocol:

• Positive control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
• Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
• Cells were incubated for 30 min on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
• Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Propidium Iodide (PI) was added to discern live cells from dead cells.
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Notes: - The data displayed is gated on PI negative cells.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CDH6

Antigène: CDH6 (K-Cadherin (CDH6))

Autre désignation: K Cadherin

Sujet:

Synonyms: CAD6, KCAD, Cadherin-6, Kidney cadherin, K-cadherin, CDH6

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

ID gène: 1004

UniProt: P55285

Pathways: Cell-Cell Junction Organization