Actin anticorps

N° du produit ABIN7223846

L’anticorps anti-Actin Polyclonal Lapin est utilisé pour la détection de Actin dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, IF et ELISA.

Aperçu rapide pour Actin anticorps (ABIN7223846)

Antigène: Actin

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Détail du produit anti-Actin anticorps

Fonction: Rabbit Anti-Actin-α/γ Polyclonal Antibody

Specificité: Actin-α/γ Polyclonal Antibody detects endogenous levels of Actin-α/γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human Actin-alpha/gamma around the non-phosphorylation site of Y55/53

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur Actin

Antigène: Actin

Autre désignation: Actin-alpha/gamma

Sujet: ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTG1, ACTB, ACTG, Actin, cytoplasmic 2, Gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG, Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin,The product encoded by ACTA1 (actin, alpha 1, skeletal muscle) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in ACTA1 cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Poids moléculaire: 45kD

ID gène: 58, 70, 71

UniProt: P68032, P63261, P63267, P68133