PRKAA1/PRKAA2 anticorps (pThr172, pThr183)

N° du produit ABIN7201115

Cet anticorps Lapin Polyclonal détecte spécifiquement PRKAA1/PRKAA2 dans WB, IHC, IF et ELISA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Porc, Singe et Penaeus japonicus.

Aperçu rapide pour PRKAA1/PRKAA2 anticorps (pThr172, pThr183) (ABIN7201115)

Antigène: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Reactivité: Humain, Souris, Rat, Porc, Singe, Penaeus japonicus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAA1/PRKAA2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Détail du produit anti-PRKAA1/PRKAA2 anticorps

Épitope: pThr172, pThr183

Fonction: Rabbit Anti-AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

Specificité: Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human AMPKalpha1/2 Phospho-Thr183/172

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,Not yet tested in other applications,

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur PRKAA1/PRKAA2

Antigène: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Autre désignation: AMPKalpha1/2

Sujet: PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA2, AMPK,Protein kinase AMP-activated catalytic subunit alpha 1 encoded by PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Poids moléculaire: 63kD

ID gène: 5562, 5563

UniProt: Q13131, P54646