CDCA3 anticorps (AA 190-270)

N° du produit ABIN7214118

Cet anticorps anti-CDCA3 Polyclonal Lapin (ABIN7214118) détecte spécifiquement CDCA3 dans WB, IHC, ELISA et IF. L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour CDCA3 anticorps (AA 190-270) (ABIN7214118)

Antigène: CDCA3 (Cell Division Cycle Associated 3 (CDCA3))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDCA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-CDCA3 anticorps

Épitope: AA 190-270

Fonction: Rabbit Anti-CdcA3 Polyclonal Antibody

Specificité: CdcA3 Polyclonal Antibody detects endogenous levels of CdcA3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the C-terminal region of human CdcA3 at AA range: 190-270

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur CDCA3

Antigène: CDCA3 (Cell Division Cycle Associated 3 (CDCA3))

Autre désignation: CdcA3

Sujet: CDCA3, C8, GRCC8, TOME1, Cell division cycle-associated protein 3, Gene-rich cluster protein C8, Trigger of mitotic entry protein 1, TOME-1Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. CdcA3 (Cell division cycle-associated protein 3), also known as TOME1 (Trigger of mitotic entry protein 1) and GRCC8 (Gene-rich cluster protein C8), is a 268 amino acid cytoplasmic protein that is required for entry into mitosis. By participating with E3 ligase complexes, CdcA3 is involved in ubiquination and degradation of the mitosis-inhibitory kinase Wee 1, leading to the dephosphorylation of Cdc2 in the late G2 phase. CdcA3 itself is targeted for destruction by anaphase promoting complex (APC) in the G1 phase of the cell cycle, then allowing for Wee 1 accumulation during interphase.

Poids moléculaire: 28kD

ID gène: 83461

UniProt: Q99618