DBF4 anticorps (AA 10-90)

N° du produit ABIN7214522

Cet anticorps Lapin Polyclonal détecte spécifiquement DBF4 dans WB, ELISA et IHC (p). Il présente une réactivité envers Humain, Souris et Singe.

Aperçu rapide pour DBF4 anticorps (AA 10-90) (ABIN7214522)

Antigène: DBF4 (DBF4 Homolog (DBF4))

Reactivité: Humain, Souris, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DBF4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DBF4 anticorps

Épitope: AA 10-90

Fonction: Dbf4 Polyclonal Antibody

Specificité: Dbf4 Polyclonal Antibody detects endogenous levels of Dbf4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from the N-terminal region of human Dbf4 at AA range: 10-90

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur DBF4

Antigène: DBF4 (DBF4 Homolog (DBF4))

Autre désignation: Dbf4

Sujet: Rabbit Anti-Dbf4 Polyclonal Antibody,DBF4, ASK, DBF4A, ZDBF1, Protein DBF4 homolog A, Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1,The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.,Protein DBF4 homolog A

Poids moléculaire: observerd band 77kDa

ID gène: 10926

UniProt: Q9UBU7

Pathways: Mitotic G1-G1/S Phases, DNA Replication